@article{184686,
  author = {Shannon M. Hoffman and Maria Alvarez and Gilad Alfassi and Dmitry M. Rein and Sergio Garcia-Echauri and Yachin Cohen and Jos{\'e} L. Avalos},
  title = {Cellulosic biofuel production using emulsified simultaneous saccharification and fermentation (eSSF) with conventional and thermotolerant yeasts},
  abstract = { Future expansion of corn-derived ethanol raises concerns of sustainability and competition with the food industry. Therefore, cellulosic biofuels derived from agricultural waste and dedicated energy crops are necessary. To date, slow and incomplete saccharification as well as high enzyme costs have hindered the economic viability of cellulosic biofuels, and while approaches like simultaneous saccharification and fermentation (SSF) and the use of thermotolerant microorganisms can enhance production, further improvements are needed. Cellulosic emulsions have been shown to enhance saccharification by increasing enzyme contact with cellulose fibers. In this study, we use these emulsions to develop an emulsified SSF (eSSF) process for rapid and efficient cellulosic biofuel production and make a direct three-way comparison of ethanol production between~<i>S. cerevisiae</i>,~<i>O. polymorpha</i>, and~<i>K. marxianus</i>~in glucose and cellulosic media at different temperatures. },
  year = {2021},
  journal = {Biotechnology for Biofuels },
  url = {https://biotechnologyforbiofuels.biomedcentral.com/articles/10.1186/s13068-021-02008-7?fbclid=IwAR1txMrE8dfmakegNgh-ndvfmsk1MlOV5grjuNmffh9CWzB-TqSkbrLTTZg},
  language = {eng},
}